Purification and Mechanism of Action of Macromomycin1

نویسندگان

  • Thomas H. Sawyer
  • Archie W. Prestayko
  • Stanley T. Crooke
چکیده

possible that contaminants in the preparation may have contributed some of the effects observed. The present study describes the purification of MCR to a homogeneous single component as demonstrated by poly acrylamide gel electrophoresis and NH@-terminaIamino acid analysis and presents preliminary information on the ability of MCR to degrade PM2 DNA in vitro and DNA within Novikoff hepatoma cells.

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Proteins Separation and Purification Methods with Focus on Chromatography: a Review Study

  Before describing the structure and mechanism of action of a protein, it must first be subject to purification procedure. Protein purification is a set of processes in which one or a small number of proteins are purified from a complex compound that may be a complete cell, tissue, or organism. Understanding the functions, structural properties, and interactions of the protein are directly re...

متن کامل

Efficacy of different antiemetics with different mechanism of action on xylazine induced emesis in cats

Xylazine hydrochloride, α2 adrenoceptor agonist, is an analgesic, sedative, tranquilizer, and muscle relaxant agent in veterinary medicine which is mostly used as in pre-anesthesia in veterinary medicine. One of the main adverse effects of xylazine which limits application of this medication in small animal veterinary practice (mostly in cats) is nausea and vomiting which can end up with aspira...

متن کامل

همسانه‌سازی و بیان ایمونوتوکسین اونتاک به صورت هیبریدی با دنباله اینتئینی

Introduction: Inteins (INT) are internal parts of a number of proteins in yeast and some other unicellular eukaryotes, which can be separated from the immature protein during protein splicing process. After identifying the mechanism of intein action, applications of these sequences are be considered in the single- step purification of recombinant proteins and different intein tags were develope...

متن کامل

Study on Antinociceptive Activity of Different Extracts of Peganum Harmala and Possible Mechanism of Action

The seeds of Peganum harmala L. ( Pgh ) (Zygophyllaceae) have been used in Moroccan traditional medicine for treatment of a various diseases and to relieve dolorous process. The major objective of this paper was to investigate the analgesic effect of ethyl acetate (EAE), butanolic (BE) and aqueous (AqE) extracts of seeds of Peganum harmala and elucided the possible underlying action mechani...

متن کامل

Hepatoprotective Activity of Camellia Sinensis and Its Possible Mechanism of Action

The present study appraised the hepatoprotective activity of aqueous extract of Camellia sinensis leaves and its possible mechanism of action against carbon-tetrachloride-induced liver damage. Liver damage was induced by   intraperitoneal administration of equal mixture of carbon tetrachloride   and olive oil (50 % v/v, 0.5 ml/kg ) in male Wistar rats (150-220g) once daily for...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2004